Fig. 3: Substrate synthesis from PET plastic waste for bioremediation.
From: A biocompatible Lossen rearrangement in Escherichia coli
a, Synthesis of the Lossen rearrangement substrate from PET waste and PET bioremediation strategy via auxotroph recovery. b, Growth curves from 96-well plate experiments during auxotroph rescue. Error bars are omitted for clarity. c, A photograph of auxotroph rescue experiments using a PET-derived substrate. d, Substrate depletion (grey) and dry cell weight (d.c.w.) production (blue) during growth experiments in Falcon tubes using 1 µM PET-1. A conversion factor of 0.33 grams per litre per OD600 was applied. All data are shown as triplicate experiments to one standard deviation. (i) NaOH, EtOH/H2O, reflux, 10 h. (ii) T3P, iPr2NEt, H2NOPiv·TfOH, tetrahydrofuran, 0 °C to room temperature, 16 h.
