Extended Data Fig. 4: 2D [15N-1H]- HMBC spectra provide evidence for His protonation at low pH.
From: The biophysical requirements that govern the efficient endosomal escape of designed mini-proteins
a-f, [15N-1H] heteronuclear multiple bond quantum coherence spectroscopy (HMBC) at pH 7.5, 6.5, 5.5, 5.0, 4.5, and 3.5 provides the chemical shifts of 15N and 1H atoms in His residues in ZF5.3.
