Extended Data Fig. 6: Additional data in support of Fig. 5.
From: The biophysical requirements that govern the efficient endosomal escape of designed mini-proteins
Deconvoluted mass spectra of HPLC-purified a, BBA5.3 and b, Rho-BBA5.3 c, AF488-BBA5.3. d, Overlay of the wavelength-dependent CD spectra of BBA5.3 at concentrations between 50 and 125 µM in a Resuspension Buffer without Zn(II) containing 20 mM Tris, 150 mM KCl, and 1 mM TCEP at 37 °C. e, Plot of the mean residue ellipticity at 222 nm of 125 µM BBA5.3 in Resuspension Buffer without Zn(II) at pH 7.5 (teal) or pH 4.5 (red) at every 2 °C between 5 °C and 95 °C. f, Wavelength-dependent CD spectra of 125 µM (0.3 mg/mL) BBA5.3 at pH 7.5 and 4.5 in Resuspension Buffer without Zn(II) at 37 °C. g, Wavelength-dependent CD spectra of BBA5.3 (125 µM) before and after being heated from 5 to 95 °C. The sequences of h, Rho-BBA5.3 and i, AF488-BBA5.3 prepared using solid phase synthesis. j, Flow cytometry histograms for Lissamine rhodamine B signal from Saos-2 cells treated with media (black), Rho-ZF5.3 (pink), or Rho-BBA5.3 (blue) at specified concentrations.
