Extended Data Fig. 7: LC-HRMS analysis of ZF5.3 and BBA5.3 integrity post-delivery into Saos-2 cells.
From: The biophysical requirements that govern the efficient endosomal escape of designed mini-proteins
a, Primary sequences of dBio-ZF5.3 and dBio-BBA5.3, each of which carries a desthiobiotin affinity tag. b, m/z values of dBio-ZF5.3 (pink) or c, dBio-BBA5.3 (blue) eluted from Streptavidin-conjugated magnetic beads in vitro (dotted), extracted from Saos-2 cells postdelivery (solid), and extracted ion chromatogram for starred m/z value for an in vitro sample (dotted), post-delivery sample (solid), non-treated in vitro sample (dotted black), and a non-treated post-delivery sample (solid black).
