Extended Data Fig. 8: Confocal images and extent of colocalization of Saos-2 cells expressing GFP-tagged organelle markers with either Rho-ZF5.3 or Rho-BBA5.3.
From: The biophysical requirements that govern the efficient endosomal escape of designed mini-proteins
Representative confocal images of Saos-2 cells indicating the extent of colocalization between the signals due to a, Rho-ZF5.3 (magenta) or b, Rho-BBA5.3 and GFP organelle markers (green) for early endosomes (Rab5), late endosomes (Rab7), lysosomes (Lamp1), and mitochondria (PDH). Regions of the cell in which the magenta and green signals overlap appear white. Scale bars = 10 μm. c, Plot illustrating the calculated Manders’ overlap correlation coefficients (mean ± SEM) for each organelle for two biological replicates. Outliers were removed using the ROUT method (Q = 0.1%). For Rab5 n = 83 and 48 cells, Rab7 n = 92 and 55 cells, Lamp1 n = 114 and 88 cells, and for PDH n = 68 and 93 cells for Rho-ZF5.3 and Rho-BBA5.3 respectively.
