Extended Data Fig. 4: Dual N- and C-terminal labeling of GS-MBP-TeCH using eSrtA and MccB/subtiligase. | Nature Chemistry

Extended Data Fig. 4: Dual N- and C-terminal labeling of GS-MBP-TeCH using eSrtA and MccB/subtiligase.

From: Engineered reactivity of a bacterial E1-like enzyme enables ATP-driven modification of protein and peptide C termini

Extended Data Fig. 4

(a) Scheme for dual N- and C-terminal label of GS-MBP-TeCH with MccB/subtiligase and eSrtA. The magenta circle represent azidoAla and the cyan circle represents 5-FAM. (b) Telescoping one-pot dual labeling of GS-MBP-TeCH with eSrtA and MccB/subtiligase. (c) Concurrent one-pot dual labeling of GS-MBP-TeCH with eSrtA and MccB/subtiligase.

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