Extended Data Fig. 2: MccB homologs from L. johnsonii and H. pylori can be used in combination with subtiligase for enzyme-catalysed expressed protein ligation. | Nature Chemistry

Extended Data Fig. 2: MccB homologs from L. johnsonii and H. pylori can be used in combination with subtiligase for enzyme-catalysed expressed protein ligation.

From: Engineered reactivity of a bacterial E1-like enzyme enables ATP-driven modification of protein and peptide C termini

Extended Data Fig. 2

(a) LjMccB- and subtiligase-catalysed ATP-dependent peptide ligation of Ala-Phe (left) or AFAGAGS-azAla (right) to GFP- LjTeCH. (b) HpMccB- and subtiligase-catalysed ATP-dependent peptide ligation of Ala-Phe (left) or AFAGAGS-azAla (right) to GFP-HpTeCH.

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