Extended Data Fig. 8: Investigations into the substrate selectivity of SacE.
From: Pseudokinases can catalyse peptide cyclization through thioether crosslinking
(a) Analysis of proton exchange through enzymatic ring opening and recylization in D2O. (i), treatment of 4 (top, control) with SacE (bottom); and (ii), treatment of 5 (top, control) with SacE (bottom). (b) Analysis of 7 production based on NEM derivatization. (i), treatment of 4 (top, control) with SacE (bottom); and (ii), treatment of 5 (top, control) with SacE (bottom) in the presence of NEM, respectively. In panels (a) and (b), for HR-MS analysis of 4 and 5, the dashed lines indicate the monoisotopic peaks of ESI m/z [M + 4H]4+ (calculated 1426.2039. See Supplementary Figs. 43 for observed details and errors). Related experiments were performed >= 3 times (with >= 2 parallel samples for each).
