Fig. 1: Indirect observation of (5′-CAG-3′)31 condensates by solvent detection.
From: Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates
a, 1D 1H-NMR spectrum of the aromatic and amino region of (5′-CAG-3′)31 (150 μM) at 25 °C (left panel), at 55 °C (central panel) and after recooling to 25 °C (right panel) in presence of an agarose meshwork (0.5%, w/v) and 50 mM MgCl2. Assignments indicate aromatic 1H resonances of adenine (A), guanine (G) and cytosine (C) and are given within the spectrum. b, Normalized integral of bulk H2O as a function of offset saturation in presence of (5′-CAG-3′)31 at 25 °C (left panel), at 55 °C (central panel) and after recooling to 25 °C (right panel), shown in blue (25 °C) or orange (55 °C). For comparison, the reference profile of the agarose meshwork was recorded at 25 °C (left and right panels) and at 55 °C (central panel), shown in black. Offset saturation occurred with a B1 amplitude of 250 Hz. c, Net effect of (5′-CAG-3′)31 on the offset saturation of solvent water, determined via subtraction of the relative water integrals shown in b. a–c, All experiments were performed at 700-MHz B0 field strength.
