Extended Data Fig. 8: 1D 1H NMR integral analysis of (5′-CAG-3′)31 upon condensation.
From: Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates
a Spectral area of observable aromatic and amino resonances of (5′-CAG-3′)31 (150 μM) under monophasic conditions (25 °C) and biphasic conditions (55 °C) in presence of 0.5% agarose (w/v) and 50 mM MgCl2. b and c Absolute integrals of the spectral area shown in a under monophasic (25 °C) and biphasic (55 °C) conditions at different concentrations of overall RNA (left panel each). Right panel: Fraction of (5′-CAG-3′)31 in dense and dilute phase under biphasic conditions, calculated by normalization of the absolute integral under biphasic conditions (dilute phase RNA) to the respective integral under monophasic conditions (total RNA). Condensed Fraction: 1-Dilute Fraction. d Partition coefficient of (5′-CAG-3′)31 at 50 mM MgCl2 (b) and at 30 mM MgCl2 (c) upon phase separation at different RNA concentrations. Partition coefficient was determined by the ratio of fraction of (5′-CAG-3′)31 in dense phase and fraction of (5′-CAG-3′)31 in dilute phase, shown in b and c, right panel each.
