Extended Data Fig. 9: Condensation of CAG repeat-expansion RNA at varying CAG repeat number.
From: Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates
a Characterization of CAG repeat-expansion RNA condensates via CONDENSE-MT as a function of number of CAG repeats and molar concentration for CAG repeat RNA. All data, including the associated errors, are also listed in Extended Data Table 1. b Population of condensed RNA, detected via CONDENSE-MT (shown in a) as a function of RNA concentration (mg/mL) for CAG repeat RNA at varying number of CAG repeats. Molar concentration is indicated by marker size, CAG repeat number is indicated by color. c Global fit for the tumbling rate of condensed water via R2,water (obtained from CONDENSE-MT, Fig. 4) and R1,water (measured via inversion recovery) as a function of Pcondensate of condensed (5′-CAG-3′)31 (at molar concentrations ranging from 37.5 μM to 150 μM, determined in Fig. 4), based on dipole-dipole nuclear spin relaxation (Bloembergen-Purcell-Pound theory)39,41,43. The obtained tumbling rate of water bound and the corresponding population of water-protons in a (5′-CAG-3′)31 condensate are given in the upper left. A detailed description of the formulas used for this fit is given in the Supplementary Note 5.
