Fig. 2: RNA condensate characterization via multi-step multi-parameter global fitting of MT data (CONDENSE-MT).
From: Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates
a, Comparison of water-detected MT profiles of aqueous buffer, in presence of 0.5% (w/v) agarose and in presence of (5′-CAG-3′)31 condensates embedded in agarose. MT profiles were recorded at 55 °C in presence of 50 mM MgCl2. b, Schematic representation of the equilibrium chemical exchange model used between the agarose meshwork, the semi-solid RNA condensate and solvent H2O. P, population; k, exchange rate constant. c, Water-detected MT profiles of the agarose meshwork (0.5% w/v, left, indicated in blue) and of (5′-CAG-3′)31 condensates (150 μM) embedded in agarose (right, indicated in orange) at different B1 field strengths (given in Hertz for each profile). d, MT effect of the agarose meshwork in absence of (5′-CAG-3′)31 (black) and biphasic (5′-CAG-3′)31, reconstituted in agarose (orange), for different B1 field strengths given above in Hertz for each panel. Net effect of (5′-CAG-3′)31 (obtained via subtraction) is shown as orange areas.
