Fig. 3: Characterization of the agarose meshwork.
From: Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates
a, Net MT effect of agarose (compared with aqueous buffer) at different concentrations of agarose at 250 Hz offset saturation. Chemical shift centre of semi-solid agarose was determined by interpolation, assuming symmetry of the agarose resonance, and is indicated in the panel below. Areas corresponding to direct self-saturation of water and to relayed saturation from agarose are indicated. CS, chemical shift. b, Multi-parameter global fitting of the water-detected MT profile of 0.5% (w/v) agarose at 55 °C. Experimentally recorded MT profile is shown as a scatter plot; theoretical prediction after computational optimization is given as dashed line. The MT profile at spectral region close to the H2O resonance (4.7 ppm) is shown enlarged below. Saturation amplitudes (B1) are given in Hertz. Parameter errors were estimated from the diagonal elements of the variance–covariance matrix resulting from fitting experimental data to the CONDENSE-MT model. Fit deviation indicates overall deviation of experimental datapoints from the model prediction after computational optimization.
