Fig. 5: Effect of CAG repeat number on CAG condensates.
From: Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates
a, Three-dimensional comparison of CAG-condensate parameters (T2,condensate, R2,water and Pcondensate) for CAG condensates as a function of CAG repeat number (indicated by colour). Condensates were reconstituted across a variety of molar input concentrations of the respective RNA (Extended Data Table 1). b, Comparison of T2,condensate with Pcondensate as a function of CAG RNA length. c,d, Comparison of Pcondensate (c) and T2,condensate (d) as a function of CAG repeat number at 100 μM RNA concentration. a–d, All parameters were determined using CONDENSE-MT. Error bars indicate parameter uncertainties, estimated from the diagonal elements of the variance–covariance matrix resulting from fitting experimental data to the CONDENSE-MT model, and are centred around the resulting parameter value after computational optimization ± parameter uncertainty. e, Direct comparison of \(R_{{2,{\rm{H}}_{2}{\rm{O}}}}\) with Pcondensate as a function of RNA length. Linear trendlines indicate increase of \(R_{{2,{\rm{H}}_{2}{\rm{O}}}}\) as function of Pcondensate. Datapoints with Pcondensate < 0.025% were excluded for the trendline. f, Ratio of protons (H2O/RNA) in CAG condensates as a function of RNA length, obtained from individual calculation of the proton ratio (H2O/RNA) across multiple Pcondensate datasets (shown in a) for each RNA length, varying in their molar RNA concentration. Calculations were performed using all datasets with a Pcondensate > 0.025%. Lower water content at increasing RNA length is indicated by dashed trendline. Detailed information is given in the Supplementary Note 5. g, Scheme of RNA condensation as a function of the number of CAG repeats. Variations in water content of RNA condensates is indicated by the number of water symbols (blue circles) per RNA droplet. Mg2+ is schematically indicated by green dots.
