Extended Data Fig. 1: Establishment of (5′-CAG-3′)31 condensates as a model system.
From: Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates
a Optical density at 600 nm of (5′-CAG-3′)31 (40μM) as a function of temperature and MgCl2 concentration. All experiments were performed in three independently prepared and measured replicates. Error bars indicate standard deviation from the average of the three replicates. b Light microscopy of (5′-CAG-3′)31 (37.5μM) in presence of 50 mM MgCl2, at 25 °C and after heating to 55 °C. Scalebar indicates 2 μm. c Incubation of biphasic (5′-CAG-3′)31 (150 μM, at 50 mM MgCl2) in 3 mm NMR tubes in a temperature-controlled water bath for 2.5 h at 55 °C in absence (left) and in presence (right) of a 0.5% (w/v) agarose meshwork.
