Fig. 4: Functional relevance of IGF1RN724D in the IGF1 signalling pathway.
From: Giant tortoise genomes provide insights into longevity and age-related disease
a, Alignment of IGF1R around residue p.N724 in C. abingdonii, A. gigantea and other representative species. The predicted electrostatic surfaces of human (top right) and modelled C. abingdonii (bottom right) IGF1R around the same residue are shown for comparison. Negatively charged areas are depicted in red, while positively charged areas are depicted in blue. b, Western blot analysis and densitometry quantification of the phospho-IGF1R (pIGF1R)/total IGF1R ratio at 5, 10 and 20 min intervals after IGF1 addition in HEK-293T cells infected with pCDH, pCDH-IGF1RWT and pCDH-IGF1RN724D plasmids. Bars indicate means ± s.e.m. *P < 0.05, Fisher’s least significant difference test (n = 3 independent experiments).
