Extended Data Fig. 10: Method of scarless strain construction.
From: Synthetic cross-phyla gene replacement and evolutionary assimilation of major enzymes
The pgi swap strains were constructed as shown. The tpiA swap strains used a construct lacking the foreign gene start homology, I-SceI site, and antibiotic cassette; double stranded DNA breaks were induced by CRISPR-targeting to native gene sequence, obviating the need for antibiotics.
