Extended Data Fig. 3: Orthogene impact on strain fitness.
From: Synthetic cross-phyla gene replacement and evolutionary assimilation of major enzymes
a, Growth rates of various pgi- and tpiA-swap evolved strains before and after knockout of the orthogene. b, Enzyme activity levels of various strains, determined by colorimetric assay. No bar indicates an activity level below detection of the assay. c, Growth rates at various AHL concentrations of pgi or tpiA knockout strains containing plasmids with AHL-inducible expression of the H. sapiens pgi or tpiA. Growth rates higher than knockout levels even with no AHL induction may be due to leaky plasmid expression. In all panels strain names correspond with those given in the Supplementary Dataset containing DNA sequencing data, and error bars represent standard deviation from quadruplicate measurements. Orthogene-assimilation failures: PaePgi 26.93, HsaPgi 17.83, HsaTpi 8.34. Orthogene-assimilation successes: VchPgi 29.76, BmePgi 13.86, HsaPgi 20.71, VchTpi 25.65, BmeTpi 13.105, PaeTpi 23.107, HsaTpi 3.96.
