Extended Data Fig. 2: Flow cytometry analysis of the SARS-CoV-2 RBD-hFc binding to 293T cells expressing different bat ACE2 orthologs. | Nature Ecology & Evolution

Extended Data Fig. 2: Flow cytometry analysis of the SARS-CoV-2 RBD-hFc binding to 293T cells expressing different bat ACE2 orthologs.

From: ACE2 receptor usage reveals variation in susceptibility to SARS-CoV and SARS-CoV-2 infection among bat species

Extended Data Fig. 2

293T cells expressing bat ACE2 orthologs were incubated with 5 μg/ml of recombinant SARS-CoV-2 RBD-hFc protein at 37 °C for 1 hour, and then washed and incubated with a Alexa Fluor 488 conjugated secondary antibody recognizing human IgG Fc. Histogram charts were generated by FlowJo with mock cells (sample 47) as negative control.

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