Extended Data Fig. 9: Resampling OUwie estimates for all species, Annelida, Mollusca, and Chordata.
From: Fertilization mode drives sperm length evolution across the animal tree of life

We used repeated resampling to validate the use of 100 SIMMAPs to estimate the evolutionary rate (θ) and evolutionary optima (σ2) of sperm length for external fertilizers (blue), spermcasters (purple), and internal fertilizers (red). We conducted 1000 iterations of resampling per each sample size of 5 to 100 estimates from our models for all species (a,e), Annelida (b,f), Mollusca (c,g), and Chordata (d,h). Generating a measure of variance in our estimate of the mean and standard deviation for both the evolutionary rate (θ) and evolutionary optima (σ2). Graphs illustrate the coefficient of variation (CV) for of these parameters for each fertilization mode.