Extended Data Fig. 4: Evolutionary reconstruction and domain architecture conservation.

a, Species tree of eukaryotes used in the ancestral reconstruction analysis, with branch lengths calibrated to the gain/loss rates of Pfam domains (see Methods). Available in Supplementary Table 1. b, Conservation of archetypical protein domain architectures across orthogroups, in acetylases, deacetylases, methyltransferases, demethylases, remodellers and chaperones. In each heatmap, we indicate the fraction of genes within an orthogroup (rows) that contain a specific protein domain (columns). Domains in bold are catalytic (black) or reader (purple) functions. At the right of each heatmap, we summarize the presence/absence profile of each orthogroup across eukaryotic lineages (as listed in Fig. 1a).