Fig. 1: Transcriptomic classification of cell types in ant brains.

a, The four adult phenotypes of M. pharaonis and a schematic overview of the overall experimental design. Four to five biological replicates for each adult phenotype were prepared for snRNA-seq. For a single biological replicate of an adult phenotype, nuclei for snRNA-seq were isolated from a pool of 30 to 50 whole brains. b, UMAP plot of the 43 cell clusters generated by grouping the 206,367 nuclei obtained from brains of workers, queens, gynes and males. Each dot represents one nucleus. See legend for numerical and colour coding. See also Supplementary Data 1 for the number of nuclei per cluster in each replicate of the four adult phenotypes.