Extended Data Fig. 4: Tajima’s D across major venom regions in CV2 and CO2 populations.
From: The roles of balancing selection and recombination in the evolution of rattlesnake venom
Tajima’s D across SVMP (a), SVSP (b), and PLA2 (c) regions in CV2 and CO2 populations. Top panels show sliding window Tajima’s D estimates in 100 kb (lines) and 10 kb (points points) windows. Venom gene regions are shaded in grey. Middle panels in a and b show zoomed in venom gene regions with sliding window estimates in 10 kb (lines) and 1 kb (points) windows. Middle panels in c show estimates in 1 kb (lines) and 250 bp (points) windows. Gaps in lines represent windows where there was insufficient data to calculate a mean estimate. Dark blue segments show the locations of venom genes in each region, and the non-venom homologue PLA2gIIE is shown in light purple in c. Regional variation in the presence of CNVs (% CNV) in CV2 (orange dashed line) and CO2 (dark blue line) is shown below venom region scans. Individual genotypes in detected CNVs were masked. Bottom panels show distributions of chromosome-specific and non-venom homologue (NV) backgrounds compared to values in each venom gene region, with boxplots showing the median (horizontal lines), interquartile (box limits), and range (whiskers). Asterisks indicate significant differences between venom gene regions and chromosome backgrounds and non-venom homologues based on two-tailed Welch’s two-sample t-tests and n = 2,293, 2,068, and 1,272 10 kb sliding windows for SVMP, SVSP, and PLA2 comparisons, respectively (*P < 0.05; **P < 0.001). Exact P-values for comparisons can be found in Supplementary Table 5.
