Extended Data Fig. 8: Phenotypes of the scFcy1 and cnFcy1 mutants. | Nature Ecology & Evolution

Extended Data Fig. 8: Phenotypes of the scFcy1 and cnFcy1 mutants.

From: Asymmetrical dose responses shape the evolutionary trade-off between antifungal resistance and nutrient use

Extended Data Fig. 8

a) Growth rate for the 29 (27 of which were successfully constructed) scFCY1 variants selected in the DMS assay validation experiments. b) Representative examples of the phenotypes observed in the spot assays (Synthetic media + 194 μM 5-FC, 10-fold dilutions starting at 1 OD/ml), where S: sensitive, r: low growth and R: full resistance. c) Phenotypes of scFCY1_opt (S. cerevisiae codon optimized FCY1 at the FCY1 locus), cnFCY1_opt (C. neoformans codon optimized FCY1 at the S. cerevisiae FCY1 locus) compared to the parental strain (BY4742) and the deletion mutant (Δfcy1). d) Comparison of growth rate between orthologous variants in 5-FC media. Spearman’s rank correlation is shown (n = 22 pairs). Variants are colored by the position along the trade-off of the scFCY1 variant. e) Comparison of 5-FC media growth rate between orthologous variants. Spearman’s rank correlation is shown (n = 22 pairs). f) Changes in growth rate in SC + 12 μM 5-FC and SC-Ura + 84 μM cytosine between scFcy1 and cnFcy1 variants. Spearman’s rank correlation is shown (n = 22 pairs).

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