Extended Data Fig. 4: Coding mutations in HOXD11B cause length changes in stickleback spines in a second anadromous Gasterosteus population.
From: Evolution of stickleback spines through independent cis-regulatory changes at HOXDB
a. X-rays of an uninjected sibling control RABS Gasterosteus (top) and a RABS Gasterosteus that was injected at the single cell stage with Cas9 and an sgRNA targeting the coding region of HOXD11B (bottom). Scale bar is 5 mm. b. Quantification of spine length changes. In the box and whisker plot: center line, median; box limits, interquartile range; whiskers, 1.5x interquartile range; each measurement is represented by a single point (circle for wild type and triangle for mutant). DS1 and DS2 were not significantly different between controls and HOXD11B mutant fish. DSL and AS were significantly longer in the F0 mutants compared to the controls (two-tailed t-test Bonferroni-corrected at α = 0.05, DSL padj = 4E-13, AS padj = 2E-07, n = 38 injected and n = 30 control from 3 clutches combined). The y-axis is the residual after accounting for the standard length of fish.
