Extended Data Fig. 9: Targeting of AxE in an anadromous Gasterosteus population also causes length changes in DSL and AS.
From: Evolution of stickleback spines through independent cis-regulatory changes at HOXDB
a. Representative uninjected LITC sibling control fish (top) and injected AxE F0 mutant (bottom). Scale bar is 5 mm. b. Quantification of spine length difference. In the box and whisker plot: center line, median; box limits, interquartile range; whiskers, 1.5x interquartile range; each measurement is represented by a single point (circle for wild type and triangle for mutant). The residual after adjusting for standard length is on the y-axis and the spines ordered from anterior to posterior are on the x-axis. DS1 and DS2 do not show a significant difference in length between control and injected. DSL and AS were significantly longer in the injected compared to the control (two-tailed t-test Bonferroni-corrected at α = 0.05, DSL padj = 3E-04, AS padj = 0.02 n = 32 control and n = 24 injected).
