Extended Data Fig. 3: Embryonic expression of Gasterosteus HOXDB genes.
From: Evolution of stickleback spines through independent cis-regulatory changes at HOXDB
In situ hybridization of Gasterosteus aculeatus embryos at Swarup stage 19/20 a. HOXD4B probe. The arrowheads point to the hindbrain, the asterisk indicates the neural tube, and the arrows point to the anterior somites; b. HOXD9B probe; c. HOXD11B probe. d. eGFP expression at embryonic Swarup stage 19–20 in the tailbud somites from the reporter gene integrated upstream of HOXD11B in low-spine Gasterosteus (Fig. 1). The eGFP pattern recapitulates the in situ hybridization results for HOXD11B in panel C. Dotted circle indicates the location of the eye. Scale bar is 1 mm.
