Extended Data Fig. 6: Evidence of positive selection in domains of Hex110 and ApoLpp.
From: Convergent and complementary selection shaped gains and losses of eusociality in sweat bees
Both Hex110 (a) and ApoLpp (b) show evidence for domain-specific positive selection associated with the origins of eusociality. Predicted binding pockets by PHYRE are shown with pink rectangles, glycosylation sites in orange squares. Pink hexagons denote MEME-identified sites that also had a mutational effect score > =1 for Hex110 and >1 for ApoLpp. In both JHBPs, MEME identifies sites in functional regions of the protein, including the receptor binding domain and predicted binding pocket for ApoLpp as well as in all three Hemocyanin domains (associated with storage functions in these proteins) and in the predicted binding pocket of Hex110. Moreover, for both proteins, we also find region-specific, faster rates of evolution on eusocial branches compared to non-eusocial outgroups in this phylogeny: the predicted binding pocket for ApoLpp and all Hemocyanin domains for Hex110. Taken together, these results suggest that positive selection shaped protein function as eusociality emerged in this group of bees.
