Extended Data Fig. 10: JH III and JH III-D3 quantification.
From: Convergent and complementary selection shaped gains and losses of eusociality in sweat bees
(a) Chemical structure of JH III in positive ionization. JH III can exist in two forms of equal m/z and produces one fragment that retains the D3 label. (b) Parent and fragment m/z values for unlabelled and labelled JH III. (c) Chromatograph of unlabelled JH III. (d) Chromatograph of labelled JH III showing similar retention time to unlabelled JH III. (e) Mass spectra of unlabelled JH III showing the expected masses based on B. (f) Mass spectra of a 50/50 mix of labelled and unlabelled JH III showing the expected masses based on B. (g) Standard curve demonstrating a range of detection of JH III. (h) Chromatograph of unlabelled JH III in the brains of bees painted with acetone (grey dotted line) or with JH III-D3 (purple solid line). (i) Chromatograph of labelled JH III in the brains of bees painted with acetone (grey dotted line) or with JH III-D3 (purple solid line). (j) Mass spectra of JH III in bee brains painted with acetone showing the expected masses based on B. (k) Mass spectra of JH III in bee brains painted with JH III-D3 showing the expected masses based on B. (l) Absolute quantification of JH III in the brains of bees painted with acetone or JH III-D3. (m) Absolute quantification of JH III-D3 in the brains of bees painted with acetone or JH III-D3. n = 3 replicates for each panel. Boxes show median and 25th and 75th percentiles. Whiskers show minimum and maximum values.
