Extended Data Fig. 2: Lipopolysaccharide biosynthesis genes affect susceptibility to pyocin R2.
From: The evolution of short- and long-range weapons for bacterial competition

Images of agar overlay assays showing pyocin R2 zones of clearing (arrows) for different LPS mutants. The strain in the overlay is indicated in the center of each plate. The source strain for the pyocin R2 is indicated at the corners. Pyocins were prepared by sterile filtering supernatant from overnight cultures. Overlays were prepared by mixing 1 mL of overnight culture with 7 mL 0.75% LB agar then thoroughly drying. a ΔwapR shows no zones of clearing. b ΔR2 ΔwbpL (the entire pyocin R2 gene cassette was first deleted from this strain, then wbpL deleted second) shows zones of clearing from WT and ΔwapR, but not when pyocin R2 is deleted. c Complementing ΔR2ΔwbpL with empty vector pSEVA-524 does not rescue clearing. d Complementing ΔR2ΔwbpL with pSEVA-524 carrying wbpL shows no clearing from WT or ΔwapR.