Extended Data Fig. 4: Embryonic phenotypes upon Cyp18a1 pRNAi.
From: A life-history allele of large effect shortens developmental time in a wild insect population
(a) Numbers (percentages) of the different phenotypic classes from one fixation analysis (upper 4 rows), in which Cyp18a1 dsRNA injected mothers were allowed to lay eggs for one day, and batches of 25-50 eggs were fixed every subsequent day for DAPI staining and microscopic analysis. Percentages of developmental arrests are indicated with dark grey background, normal development with white background. The total number of developmental arrests corresponded well to the number of unhatched eggs after the pRNAi screen for developmental time (lower two rows). In 20% of the embryos, we did not observe any start of development at all (upper row), compared to 7.8% in the control RNAi. No developmental arrests were observed upon control pRNAi. (b) Control RNAi, normal blastoderm stage. (c) Cyp18a1 RNAi. Developmental arrests at the blastoderm stage were recognized by irregular positioning of aberrant nuclei. (d) Control RNAi. Normal extending germband (fully extended in (d)). (e) Developmental arrests during germband extension were recognized by shortened and irregular germbands. (f) Control RNAi, normal dorsal closure. (g) Cyp18a1 RNAi. Embryos that were arrested during dorsal closure were still open at the dorsal side, but showed otherwise advanced development. Scale bar in (b) = 200 μm and applies to panels b-g.
