Extended Data Fig. 5: Allele frequency of F in a natural population is 0.81.
From: A life-history allele of large effect shortens developmental time in a wild insect population
Genotyping PCR (Ethidium bromide staining shown on agarose gel) of one sample of 48 beetles from the (expanded) wild population collected by Rentokil from a bakery in The Netherlands (see Methods). 31 show only a 740 bp band (homozygous F allele), 2 show only a 942 bp band (homozygous S allele), and 14 are heterozygote (both bands + a hybrid band present). Thus the allele frequency of F is 0.81, and S is 0.19. Ladder is GeneRuler 1kb plus DNA ladder (Invitrogen).
