Extended Data Fig. 1: Replicate transformation efficiency assays.

Transformation efficiency assays for CH426 (purple) and gene deletion mutants for mod3(turquoise) and mod5 (yellow). The assay was repeated three times (N = 3 biologically independent replicates) to account for day and plating effects (A-C). Each coloured dot is an independent plating count for a replicate transformation experiment, empty circles show the mean. Lower bound for the confidence interval not shown where it cannot fit onto the log scale due to being very small, or less than zero.