Extended Data Table 1 The effect of expressing AlSLR1 on the ability of stigmas to reject A. arenosa pollen in nine A. thaliana accessions differing in both the genetics of self-fertility and the phenotype resulting from attempts to restore self-incompatibility

From: A pollen selection system links self and interspecific incompatibility in the Brassicaceae

  1. a The presence (+) or absence (-) of an intact SRK gene was inferred from sequence analysis of amplified DNA sequences32,53,54.
  2. b Restoration of the self-incompatibility (SI) response was assessed by pollination assays in plants expressing A. lyrata-derived SRKb and SCRb genes for the Col-0, Ws-0, Mt-0, RLD, No, Nd-0, and C24 accessions29, in plants expressing an engineered functional SCRa gene for Wei-1 or by pollination of stigmas with A. halleri pollen for Old-130. Self-incompatibility could not be restored (-) in the Mt-0, No, and Nd-0 accessions. In the remaining accessions, pollination assays showed a robust self-incompatibility phenotype (+) which was either transient (expressed in late stage-12 and stage-13 stigmas but not in stage-14 stigmas) or stable (expressed in late stage-12, stage-13, and stage-14 stigmas).
  3. c The self-pollen rejection pathway was inferred from the results shown in (b) of attempts to restore self-incompatibility: it is operational (+) in accessions that could be reverted to self-incompatibility but is not operational (-) in accessions in which self-incompatibility could not be restored.
  4. d Mutations associated with the switch to self-compatibility in A. thaliana accessions were inferred from a combination of DNA sequencing32,53,54 as shown in (a) for SRK, genetic analysis that assessed the presence of mutations at self-incompatibility modifier loci29,30, and the success or failure of attempts to restore self-incompatibility as described in (b).
  5. e The compatible (+) and incompatible (-) responses of stage-13 stigmas towards A. arenosa pollen in wild type (WT) A. thaliana and A. thaliana:AlSLR1 T1 transgenic plants were determined in this study.
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