Extended Data Fig. 7: pou5f3 gain-of-function experiments in lamprey embryos.
From: Shared features of blastula and neural crest stem cells evolved at the base of vertebrates
(a) Western blot showing matched protein levels for gain-of-function experiments. (b) Overexpression of Xenopus pou5f3 factors in lamprey causes loss or no change of expression of multiple neural plate border and neural crest markers. Embryos are shown in dorsal view. Anterior is up and posterior is down. Asterisk denotes the injected side of the embryo. Reproducible on n = 10 embryos per time point for n ≥ 3 experiments for each panel in (a, b). Scale bar: 250 μm.
