Extended Data Fig. 10: Sequence comparisons of chordate pou5 and pou3 proteins.
From: Shared features of blastula and neural crest stem cells evolved at the base of vertebrates
(a) Alignment of Xenopus pou5 and pou3 proteins. The box on the C-terminus shows a conserved region in the transactivation domain of pou5 that is highly conserved across most vertebrates (asterisks in b). (c) Alignment of chordate pou3 and vertebrate pou5 proteins reveals a conserved potential SUMOylation site (first box) within the pou-s domain of pou3 that is absent from pou5. There are also highly conserved residues among pou3 proteins (second and third boxes) representing potential MAPK phosphorylation sites in the linker domain and pou-hd that are also absent from pou5. Full alignment is in Supplementary Fig. 2.
