Extended Data Fig. 7: Sensitivity analysis of the results of the forward genetic Wright—Fisher simulations (see Methods for details).
From: Genomic and fitness consequences of a near-extinction event in the northern elephant seal
Genetic loads in the simulated population are shown over time (measured in generations), starting from one generation before the bottleneck until the present day. Thick coloured lines represent values averaged over 100 forward genetic simulations (grey lines) and the vertical dashed red lines indicate the onset of the bottleneck. a–d, Genetic load estimates from simulations where deleterious mutations were allowed to arise only within exons, shown separately for the total load (a), the realized load (b), the inbreeding load (c) and the drift load (d). These models were based on point Ne estimates from the best-supported demographic model derived from the RAD sequencing data. e–h, Genetic load estimates incorporating uncertainty from the demographic reconstruction, shown separately for the total load (e), the realized load (f), the inbreeding load (g) and the drift load (h). For this analysis, we used Ne estimates from 100 bootstrap SFSs derived from the RAD sequencing data and allowed deleterious mutations to appear anywhere in the genome. i–l, Genetic load estimates from simulations based on the whole genome sequencing (WGS) data, shown separately for the total load (i), the realized load (j), the inbreeding load (k) and the drift load (l). For this analysis, we used point Ne estimates from the best-supported demographic model derived from the WGS data and allowed deleterious mutations to appear anywhere in the genome.
