Fig. 3: Cross-species comparison of decidual stroma.
From: Cell type and cell signalling innovations underlying mammalian pregnancy
a, Marker gene expression of endometrial stromal and decidual cell types. b, In situ hybridization (italics) and immunohistochemical (upright) localization of select markers genes from a. Cells of interest are marked by an asterisk. Scale bars, 200 μm. c, Force atlas embedding of tenrec endometrial stromal cells with RNA velocity vectors overlaid. d, Expression of select velocity-associated genes in the dS1 → dS2 transition in all cells from c, ordered by increasing velocity-informed pseudotime. e, Expression of predecidual markers in primary tenrec uterine stromal cells cultured in vitro with deciduogenic stimuli. P values reflect PyDESeq2 Wald test P values after Benjamini–Hochberg correction for multiple testing. f, Network of SAMap mapping scores between stromal cell types, with edge weight proportional to mapping scores, coloured by community as identified by Leiden clustering of the graph. Edges with scores less than 0.15 are not shown for clarity. g, Correlation heatmap of cNMF gene expression programs expressed in decidual stromal cells, with a hierarchically clustered dendrogram on top showing division into two families. The opossum eS1 cell program (non-deciduate endometrial stromal cells) is included as an outgroup. h, Tree showing inferred evolutionary relationships of decidual cell types. i, Schematic of developmental relationships between decidual cell types and corresponding gene-regulatory signatures. Silhouettes from PhyloPic under a Creative Commons licence: human, NASA (PDM 1.0); tenrec, Yan Wong (CC0 1.0); macaque, Jane Whitehouse (CC0 1.0); mouse, Michael Keesey (PDM 1.0).
