Extended Data Fig. 10: Daily activity patterns are regulated by internal circadian signalling and light.
From: Widespread temporal niche partitioning in an adaptive radiation of cichlid fishes
a, Schematic of the timeline and light cycle for the behavioural assays of N. buescheri (Neobue) and N. tretocephalus (Neotre). Note that there is a 14:10 h light:dark cycle with 3 days of light:dark, and 3 days of dark:dark. b, left: weekly speed traces (mean +/- SD) of the full 6 days of assay, right: daily average traces (mean +/- SD) for the light:dark and dark:dark periods. c, quantification of speed during the day and night for light:dark, and subjective day and subjective night for the dark:dark period, P-values were calculated by a two-sided paired t-test. d, periodograms of the light:dark and dark:dark periods for both species. PSD = power spectral density. Significant peaks are listed on top of the plot for each condition. e-g, Scatter plots of the pGLS-GWAS p-value and GLM-GWAS p-values for all SNPs associated with PC1 loadings (e), PC2 loadings (f), and total rest (g). All SNPs associated with circadian genes are labelled. Dotted lines indicated genome wide cutoffs for identification of HAVs. Statistics are derived from the fitting of a linear regression (GLM-GWAS), or linear regression using phylogenetically corrected generalised least squares (pGLS) (pGLS-GWAS).
