Extended Data Fig. 1: Biological imagery and model parameterisation experiments.
From: Critical thresholds of adult patch density and spacing during coral fertilization
(a) Scanning electron microscopy of coral egg-sperm bundles, eggs, and sperm. Parameterisation of each gamete stage and process influences the predictions from the fertilisation model. The relative sizes of gametes have been adjusted for clarity. (b) The effects of egg concentration on coral fertilisation success at two sperm concentrations, analysed with a binomial GLMM. The test was two-sided, and p-values were adjusted for multiple comparisons using the Holm-Bonferroni method. Darker points and line represent 106 sperm mL−1 (n = 18 biological replicates), and lighter points and line represent 104 sperm mL−1 (n = 19 biological replicates). Lines show the estimated mean, and shaded bands represent the 95% confidence intervals. (c) Egg ascent rates of Acropora cf. tenuis (n = 11 biological replicates) compared to bundle ascent rates of Acropora cf. tenuis (n = 13 biological replicates) previously reported in Ricardo, et al.60. (d) Fertilisation success of Acropora cf. tenuis following time from spawning (min) with a 5-min sperm contact exposure time, analysed with a binomial GLMM. The test was two-sided, and p-values were adjusted for multiple comparisons using the Holm-Bonferroni method. Darker points and line represent 7×107 sperm mL−1 (n = 24 biological replicates), and lighter points and line represent 2×106 sperm mL−1 (n = 21 biological replicates). Lines show the estimated mean, and shaded bands represent the 95% confidence intervals. Note the log scale on the x-axis.
