Extended Data Fig. 2: Validation and releases of tracer dyes to simulate coral sperm dispersion. | Nature Ecology & Evolution

Extended Data Fig. 2: Validation and releases of tracer dyes to simulate coral sperm dispersion.

From: Critical thresholds of adult patch density and spacing during coral fertilization

Extended Data Fig. 2

(a) Vertical decay of coral sperm and tracer dyes over time within a single water column. Curves represent concentration profiles for sperm (n = 20 time measurements), rhodamine WT (n = 9), and fluorescein (n = 9). Inset: Absorbance spectral profiles of coral sperm at four sperm concentrations (sperm mL−1), rhodamine WT dye at multiple concentrations, and fluorescein dye at multiple concentrations. (b) (Left) A representative example of a fluorescein dye release from a research vessel imaged with a UAV. (Right) The same image after the colour band indexing. (c) Current velocity (n = 11762) measured 1 m above the benthos along a north-facing reef slope at Wistari Reef during a ~ 4 mo. deployment. Measurements represent a time series recorded by a single tilt meter. Dotted lines represent the median and 2.5th and 97.5th percentiles. Inset: Density plot of the current velocities. Thicker bar denotes ~1 SD from the median, thinner bar denotes ~2 SDs from the median.

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