Extended Data Fig. 5: Effects of long-term N and P fertilization on microbial community diversity, composition, and structure.
From: Soil carbon sequestration enhanced by long-term nitrogen and phosphorus fertilization
a, Archaeal α-diversity (Shannon index), community structure (non-metric multidimensional scaling, NMDS based on Bray–Curtis dissimilarities), and taxonomic composition under four fertilization treatments: Nil (no fertilizer), P (phosphorus fertilization), N (nitrogen fertilization), and NP (combined N and P fertilization). b, Bacterial community. c, Fungal community. Box plots represent first and third quartiles (box), medians (central horizontal line), largest value smaller than 1.5 times the interquartile range (upper vertical line) and smallest value larger than 1.5 times the interquartile range (lower vertical line). Different letters above boxes indicate statistically significant differences among treatments (n = 4), determined using one-way ANOVA followed by Tukey’s post hoc test (two-sided; p < 0.05). Different lowercase letters indicate significant differences (p < 0.05) between fertilization treatments. Asterisks in NMDS plots indicate significant differences in microbial community structure between treatments based on PERMANOVA, with p-values adjusted using the Benjamini–Hochberg method (FDR corrected, p < 0.05). Shaded areas in NMDS plots represent 95% confidence intervals for each treatment group.
