Extended Data Fig. 5: Induction of angiogenesis in bioprinted vascular tubes.
From: Recapitulating macro-scale tissue self-organization through organoid bioprinting
a, Evolution of a printed vascular tube exposed to high VEGF concentration. Many capillaries are formed de novo while maintaining the continuous lumen. Scale bars, 250 µm. b, Evolution of a printout with straight angles showing good conservation of the printing geometry over time. Scale bar, 1000 µm. c, Complex geometries can also be printed, resulting in branched vascular networks with strong angiogenesis potential. Scale bar, 1000 µm. d, Bright-field images showing the evolution of printed vascular tube with addition of VEGF at different time points. Angiogenesis can be triggered at a desired time, promoting formation of capillaries that can connect with other tubes. Images are representative of n = 3 biologically independent experiments. Scale bar, 1000 µm.
