Extended Data Fig. 9: Quantitative real-time PCR data of three hSI organoids lines grown in SS-Hybrid50 hydrogels.
From: Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis
Quantitative real-time PCR data showing the relative expression of hSI organoids grown in SS-Hybrid50 gels for differentiated cells markers, stem cells (OLFM4, LGR5 and SOX9), Paneth cells (ATOH1, LYZ, REG3A and DEFA6), Goblet cells (MUC2 and SPINK4), Enteroendocrine cells (CHGA) and Enterocytes (ALDOB, FABP1, FABP2 and VIL1). The data were shown as mean (red line) relative expression to hSI organoids grown in Matrigel mean ± S.E.M (error bars) from three (n = 3) independent experiment (by circles, squares and triangles). Expression level was normalized to GAPDH and relative mRNA expression levels were shown as logarithmic scale (Log2). Statistical analysis was evaluated with two-ways ANOVA followed by Bonferroni post-hoc analysis and P-values were available above the data, statistically significant results were highlighted in red color (below 0.05).
