Extended Data Fig. 8: Role of crosslinking on different migration modes.
From: Self-generated gradients steer collective migration on viscoelastic collagen networks
a. Montages from wound healing assays using A431 cells plated using stencils on collagen networks pre-treated with PBS or the crosslinker glutaraldehyde. Red lines indicate the leading edge. Horizontal Scale Bar: 500μm. HH:MM. Lower Panels: Kymograph showing progression of the leading edge over time. Red lines indicate the velocity of wound closure on each side. Vertical Scale Bar: 20 hours. b. Boxplot of wound closure speed on control and crosslinked collagen gels. For a,b, representative images/data from n=7, 7 collagen gels, N=3,3 independent experiments. **p < 0.01 for Welch’s t-test (p=0.00258). c. Boxplots of relative collagen density and relaxation time (τr) following cluster removal using Trypsin/NH4OH for collagen networks treated with Lysyl Oxidase (LOX). Each dot represents one cluster that was rapidly removed. Data represents n=40, 25 clusters, N=5, 3 independent experiments. ns:p > 0.05, ***p < 0.001 for Welch’s t-test (p=0.717, 0.000550). d. Boxplots of mean instantaneous speed and coefficient of persistence for cluster migration on LOX-treated collagen networks. Data represents n=114, 42 clusters, N=6, 2 independent experiments. Control data is the same data as presented in Fig. 1f. *p < 0.05, ***p < 0.001 for Welch’s t-test (p=5.707e-6, 0.0472). e. Boxplots of relative collagen density (left) and relaxation time (τr, right) following cluster removal using Trypsin/NH4OH for collagen networks using different collagen concentration or polymerization temperature. Each dot represents one cluster that was rapidly removed. Data represents n=30, 40, 23, 18, 30 clusters, N=3, 5, 3, 2, 3 independent experiments. One-way ANOVA p=8.30e-5, 5.22e-5. **p < 0.01 for Tukey HSD post-hoc test (p=0.375, < 0.001,0.001665, < 0.001, 0.0332, 0.176,0.01, 0.539, 0.204, 0.195; p=0.27, 0.506, 0.159, < 0.001, 0.0579, 0.373, < 0.001, 0.0497, < 0.001, 0.00683).
