Extended Data Fig. 9: Single cell collagen deformation and migration.
From: Self-generated gradients steer collective migration on viscoelastic collagen networks
a. Micrographs of a primary human cancer associated fibroblast (CAF) on a fluorescent collagen network. Scale bar: 50μm. Scale vector: 0.5μm/min. HH:MM after addition of Trypsin/NH4OH. Representative image from n=18 CAFs, N=2 independent experiments. b. Boxplots comparing relative collagen density before and after CAF removal. c. Boxplot of viscoelastic relaxation time (τr) following CAF removal. For b,c: each dot represents one cluster. Data from n=39, 18 clusters/CAFs, N=5 2 independent experiments. For b,c, *p < 0.01, ***p < 0.001 for Welch’s t-test (p=6.65e-6, 1.19e-6, 0.0260). d. Micrographs from CaCo2 single cell or cluster on collagen networks. Red: migration trajectories. Scale Bar: 50μm. HH:MM. e. Overlaid migration trajectories for CaCo2 single cells and clusters, adjusted to start at the origin (0,0). f. Boxplots of mean instantaneous speed and coefficient of persistence for A431/CaCo2 clusters/single cells. For c-e, representative examples/data from n=114, 89, 69, 27 clusters/single cells, N=6, 3, 2, 2 independent experiments. A431 cluster data is same as in Fig. 1f. *p < 0.05, ***p < 0.001 for Welch’s t-test (p=2.57e-9, 3.44e-15, 5.25e-13, 0.0185). g. Overlaid migration trajectories for clusters and single cells on thin isotropic or aligned collagen networks, adjusted to start at the origin (0, 0) and rotated with respect to the collagen alignment direction. h. Boxplot of orientational index along collagen alignment direction. i. Boxplots of mean instantaneous speed and coefficient of persistence. For g-i, Data represents n=56, 86, 103, 45 clusters, N=2, 4, 2, 2 independent experiments. Data for cluster migration on thin aligned networks is same as Extended Data Fig. 5g-i. For h,i, ns=p > 0.05, ***p < 0.001 for Welch’s t-test (p=3.89e-9, 4.28e-9, 0.209, 2.30e-5, 8.74e-6, 0.000379).
