Fig. 2: Comparison of lead polymer candidates.
From: Species-agnostic polymeric formulations for inhalable messenger RNA delivery to the lung
a,b, Luminescence measurements using the lead polymer candidates. Images (a) and fold change total flux (b) of lungs 24 h after aNLuc mRNA nebulization into mice using the indicated polymers at 5 mg kg–1. ***p < 0.001 compared with all the other groups (one-way ANOVA with multiple comparisons on log-transformed data). The bars indicate the geometric mean ± standard deviation (s.d.). c, Representative microscopy of mouse lungs 4 h after nebulized delivery with the indicated polymer. FISH for aNLuc (white), Scgb1a1 marker of airway cells (green) and nuclei stained via 4′,6-diamidino-2-phenylindole (DAPI, blue). Scale bar, 15 mm. n = 2 mice per group. Larger lung areas are presented in Supplementary Fig. 8. d, Quantification of mean intensity of aNLuc-positive RNA granules in lungs from c. The bars represent mean. n = 2 mice per group. Per-cent area measurements are shown in Supplementary Fig. 9. All the p values are listed in Supplementary Table 3.
