Extended Data Fig. 7: IHC characterization of RAB5A, pCHK1 and cGAS in human DCIS and breast cancer organoids.
From: Tissue fluidification promotes a cGAS–STING cytosolic DNA response in invasive breast cancer
a. Representative (1 out of more than 20 tested) multiplex immunohistochemistry/immunofluorescence (mIHC/IF) of RAB5A, pCHK1 and DAPI in human Ductal breast Carcinoma in Situ (DCIS). A magnified image from the selected yellow boxes is shown. Scale bar 250 μm. b. Representative (1 out of more than 20 tested) multiplex OPAL immunohistochemistry/immunofluorescence (mIHC/IF) of cGAS and γH2AX in 2 distinct human DCIS. Scale bar 100 μm. c. Representative immunohistochemistry analysis of cGAS in human DCIS with invasive buds in n = 5 distinct human DCIS. A magnified image from the selected boxed area is shown. Red Arrow indicates the crescent-like, perinuclear accumulation of cGAS signal. Scale bar 80 μm. d. Representative immunohistochemistry analysis of cGAS in locally invasive human DCIS (n = 9 distinct human DCIS). Magnified images from the selected boxed area are shown. Red arrow indicates the crescent-like, perinuclear accumulation of cGAS signal, black arrows indicate the perinuclear dotted distribution of cGAS. Scale bar 40 μm. e. Representative multiplex immunohistochemistry/Immunofluorescence (mIHC/IF) of RAB5A, γH2AX, cGAS and DAPI in n = 5 patient-derived breast cancer organoids. Magnified images from the selected yellow boxes are shown. Scale bar 150 μm.
