Fig. 5: Endocytic-dependent tissue fluidification results in DNA damage.
From: Tissue fluidification promotes a cGAS–STING cytosolic DNA response in invasive breast cancer
a,b, Images of control and RAB5A-MCF10.DCIS.com monolayers (n = 3 experiments), stained with the indicated antibodies. Scale bar, 20 μm. c, The scatter plot shows the mean of 53BP1 foci per nuclei or γH2AX foci per nuclei (>150 cells in n = 3 experiments), two-tailed Mann–Whitney non-parametric test. d, Representative images of neutral comet assay in MCF10.DCIS.com monolayer cells expressing RAB5A. Control cells irradiated (IR 5 Gy) or not (CTR) are also shown. Scale bar, 100 μm. e, Quantification of DNA damage by tail moment analysis. Horizontal bars indicate the means and s.d. of from n = 2 independent experiments; >100 cells per sample were scored, one-way ANOVA.
