Fig. 5: MGV-based MMUS imaging in mouse liver.
From: Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo
a, Experimental scheme of ex vivo liver MMUS imaging. b,c, Ex vivo liver MMUS imaging (b) and SBR quantification (c) before and after fixation. MGVs, GVs or MNPs were injected into the tail vein of mice, and the liver was extracted 5 min after the initial injection. After the first round of imaging, the liver was fixed with 10% formalin for 48 h and imaged again. P = 0.000178. n = 3 animals per group. d, Experimental scheme of in vivo liver MMUS imaging. e,f, In vivo liver ultrasound images (e) and SBR quantification (f) of live animals. Three different nanomaterials (MGVs, GVs or MNPs) were injected intravenously and MMUS images were taken after 5 min. B-mode images reveal the position of the liver, AM images show the GV signal inside the liver and Δ MMUS images (parula scale) were overlapped with Doppler images (grey scale) to show the signal below the skin. P (left–right) = 0.00000012, 0.00000022. n = 5 animals per group. In b min and max on the colour bar represent between 0 and 10,000 arbitrary units; in e min and max on the parula (MMUS) and hot (AM-mode) colour bars represent between 0 and 500,000 arbitrary units, respectively, and the grey (B-mode) colour bar represent between 0 and 1,000,000 arbitrary units. Each data point represents biologically independent animals, and lines represent the median of each group. Significance was determined using multiple unpaired two-sided t-test with Welch’s correction within each group (c) and one-way ANOVA with Tukey’s multiple comparisons (f): *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
